Abstract

     The study was carried out in vitro plants of Education College Radfan affiliated University of Aden, during September 1, 2018 until February 25, 2018. The two incubation method Blotter paper and Agar plate were used to isolate local red kind of Sesamum indicum.
     The five types of fungi Aspergillus niger, Aspergillus flavus, Phoma as, Macrophomina phaseolina and Rhizopus spp belonging to different four varieties at frequencies average 15,19,10,4 and 3 respectively have been isolated by Agar plate.
     When sodium hypochlorite was used to sterilize seeds at 2% for 2 and 5 minutes, the result showed that all the above fungi exclusive Rhizopus spp and Alternaria alternate appeared at low frequencies average for A.Flavus, A.alternate, M.phaseolina and Phoma spp 1, 0.5, 2.5 and 0.5 respectively. The Blotter paper were used to isolate four fungi belonging to three kinds Aspergillus niger, Aspergillus flavus, Alternaria spp and Rhizopus sp at frequencies average 12.5, 4.25 and 3.3 respectively. The study results showed that the Agar plate outperforms the Blotter paper. 
   Sodium hypochlorite was used to sterilize seeds at 2% for 2 and 5 minutes led to  reduce the percentage of containment seeds( 16.5% and 11% respectively) compared to control 94.5% and  increased germination 95% compared to control not exceed 25%.