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E-ISSN: 2788-9327

P-ISSN: 1606-8947

Vol. 27 No. 2 (2023)

Isolation, identification, and antifungal resistance of Candida species from various samples

  • Khaled Nasher Qahtan Salem Microbiology Section, Biology Department, Faculty of Science, University of Aden
  • Abdullah Salem Ben Barakat Bawazir Microbiology Section, Biology Department, Faculty of Science, University of Aden
  • Asia Saleh Moqbel Muthana Microbiology Section, Biology Department, Faculty of Science, University of Aden
  • Asrar Mohammed Abdullah Ali Microbiology Section, Biology Department, Faculty of Science, University of Aden
  • Mariam Ali Haitham Ahmed Microbiology Section, Biology Department, Faculty of Science, University of Aden
  • Muna Yaser Ahmed Saif Microbiology Section, Biology Department, Faculty of Science, University of Aden
  • Samah Mokhtar Salem Alhaithami Microbiology Section, Biology Department, Faculty of Science, University of Aden
  • Wala Fahd Hashem Mohammed Microbiology Section, Biology Department, Faculty of Science, University of Aden
  • Zaina Mohammed Mothna Rashed Microbiology Section, Biology Department, Faculty of Science, University of Aden

University of Aden Journal of Natural and Applied Sciences, Vol. 27 No. 2 (2023), 31-10-2023 Page 233-248

DOI:

https://doi.org/10.47372/uajnas.2023.n2.a06

Abstract

This study in Aden, Yemen, collected 67 samples from patients attending hospitals and laboratories. Samples were taken from different body areas, covering various age groups and genders. Fungal infections, particularly Candida species, are a significant cause of death worldwide. The emergence of azole-resistant Candida has raised concerns and highlighted the need for better antifungal agents.
In the study, 68.66% of the collected samples contained pathogenic Candida fungi. Some samples also showed bacterial growth or both bacteria and fungi. Suspected Candida growth on corn meal agar exhibited chlamydospore formation and germ tubes on human serum. Carbohydrate fermentation tests were positive for Glucose, Maltose, Galactose, and Xylose, while Sucrose and Lactose were negative. All Candida isolates (100%) were accurately identified to the species level using CHROM agar Candida. Candida's pathogenicity was linked to various virulence factors, including hydrolytic enzyme secretion. Nystatin was the most effective antifungal agent (100% sensitivity), while Fluconazole (73.92%) and Amphotericin B (52.17%) showed varying levels of sensitivity. However, Voriconazole, ketoconazole, and Miconazole displayed high resistance rates (89.13%, 84.78%, and 80.44%, respectively) against the isolated Candida.
This study concluded that Candida spp., particularly Candida albicans, were frequently found in the samples from patients, with vaginal swaps being a common site of infection. The identification of Candida isolates to the species level using CHROM agar Candida was accurate. Nystatin, Fluconazole, and Amphotericin B were the most effective antifungal agents against Candida spp.

Keywords:
    : Candida species, Candidiasis, Antifungal resistance

How to Cite

Salem, K. N. Q., Bawazir, A. S. B. B., Muthana, A. S. M., Ali, A. M. A., Ahmed, M. A. H., Saif, M. Y. A., Alhaithami, S. M. S., Mohammed, W. F. H., & Rashed, Z. M. M. (2023). Isolation, identification, and antifungal resistance of Candida species from various samples. University of Aden Journal of Natural and Applied Sciences, 27(2), 233–248. https://doi.org/10.47372/uajnas.2023.n2.a06
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