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E-ISSN: 2788-9327

P-ISSN: 1606-8947

Vol. 23 No. 2 (2019)

Extraction and immobilization of Proteolytic Enzyme from local Yemeni Bean Seeds (Dolichos Lablab L.)

  • Maher Ali Al-Maqtari Chemistry Department, Faculty of Science, Sana'a University, Yemen
  • Khalid Mohammed Naji Chemistry Department, Faculty of Science, Sana'a University, Yemen
  • Lena Kassem Ali Faculty of Medicine, Taiz University

University of Aden Journal of Natural and Applied Sciences, Vol. 23 No. 2 (2019), 31-10-2019 Page 375-381

DOI:

https://doi.org/10.47372/uajnas.2019.n2.a09

Abstract

The aim of this study is to prepare immobilized protease with high hydrolytic activity forbiotechnological applications. Local Bean Seeds (Dolichos Lablab L.) were used for the extraction of acidprotease using tris-buffer, pH 4.5 as enzyme solvent. Free acidic protease was immobilized on entrapment in calcium alginate gel (in situ activated) by covalent binding method. Their activity and immobilization efficiency for hemoglobin hydrolysis was investigated. Temperature and pH maxima of the immobilized protease showed no changes before and after immobilization. The immobilized protease exhibited good thermal stability and re-usability.

Keywords:
    Acid protease, Yemeni Bean Seeds, Immobilization, Optimization

How to Cite

Al-Maqtari, M. A., Naji, K. M., & Ali, L. K. (2019). Extraction and immobilization of Proteolytic Enzyme from local Yemeni Bean Seeds (Dolichos Lablab L.). University of Aden Journal of Natural and Applied Sciences, 23(2), 375–381. https://doi.org/10.47372/uajnas.2019.n2.a09
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